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Integrated AFM–SECM in Tapping Mode: Simultaneous Topographical and Electrochemical Imaging of Enzyme Activity
Author(s) -
Kueng Angelika,
Kranz Christine,
Lugstein Alois,
Bertagnolli Emmerich,
Mizaikoff Boris
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200351111
Subject(s) - scanning electrochemical microscopy , atomic force microscopy , electrochemistry , chemistry , nanotechnology , glucose oxidase , microscopy , enzyme , biophysics , materials science , analytical chemistry (journal) , electrode , optics , biochemistry , organic chemistry , physics , biology
Microscopically measuring the activity of glucose oxidase (GOD) : Microfabricated difunctional AFM–SECM (atomic force microscopy–scanning electrochemical microscopy) tips with integrated micro‐ and nanoelectrodes (see picture) enable independent, simultaneous investigation of surface topography and enzymatic activity, for example, of the oxidoreductase enzyme GOD, in soft samples in AFM tapping mode.