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New Mass Spectrometric Methods for the Quantification of Protein–Ligand Binding in Solution
Author(s) -
Breuker Kathrin
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200301695
Subject(s) - chemistry , mass spectrometry , ligand (biochemistry) , protein ligand , dissociation constant , chromatography , titration , matrix assisted laser desorption/ionization , protein mass spectrometry , desorption , analytical chemistry (journal) , tandem mass spectrometry , adsorption , biochemistry , receptor
At first sight , it is not immediately discernible how molecular‐weight measurements can be used for the determination of binding constants for protein–ligand complexes. Nevertheless, successful strategies have been developed for the quantitative analysis of protein–ligand binding by mass spectrometry (see scheme), which offers the distinct advantages of speed and the potential for automation.