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Tuning PCR Specificity by Chemically Modified Primer Probes
Author(s) -
Strerath Michael,
Marx Andreas
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200290043
Subject(s) - primer (cosmetics) , computational biology , polymerase chain reaction , microbiology and biotechnology , chemistry , biology , genetics , gene , organic chemistry
Increased selectivity in PCR! Robust systems that allow the direct detection of single nucleotide variations through PCR methods will be increasingly important for rapid comparative genome analysis or mutation detection. Primer probes bearing supplemental chemical modifications (R=vinyl) significantly increase the selectivity of PCR compared to unmodified probes (R=H), even when the target sequences differ in only one nucleotide (see picture).