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Site‐Directed Alteration of RNA Sequence Mediated by an Engineered Twin Ribozyme
Author(s) -
Welz Rüdiger,
Bossmann Katrin,
Klug Christian,
Schmidt Christian,
Fritz HansJoachim,
Müller Sabine
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200250611
Subject(s) - ribozyme , rna , ligase ribozyme , mammalian cpeb3 ribozyme , computational biology , sequence (biology) , biology , genetics , hairpin ribozyme , nucleotide , gene
Repair of deletion mutations at the RNA level: A small synthetic twin ribozyme supports the insertion of four additional nucleotides into a predefined site of an arbitrarily chosen substrate RNA (see scheme). This method of RNA repair constitutes a novel approach to the therapy of medically relevant mutations.