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Magnetically Amplified DNA Assays (MADA): Sensing of Viral DNA and Single‐Base Mismatches by Using Nucleic Acid Modified Magnetic Particles
Author(s) -
Patolsky Fernando,
Weizmann Yossi,
Katz Eugenii,
Willner Itamar
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200250379
Subject(s) - nucleic acid , dna , schematic , nucleic acid thermodynamics , chemistry , base pair , microbiology and biotechnology , biology , biophysics , biochemistry , base sequence , electronic engineering , engineering
Taking DNA analysis for a spin : Amplified detection of viral DNA and single‐base mutations in nucleic acids is accomplished by the thermally cycled incorporation of biotin labels into DNA replica associated with magnetic particles, by the electrocatalyzed biochemiluminescence transduction of the DNA recognition events, and by the rotation of the magnetic particles on electrode surfaces (see schematic representation).