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Single‐Molecule‐Sensitive Fluorescent Sensors Based on Photoinduced Intramolecular Charge Transfer
Author(s) -
Sauer Markus
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200201611
Subject(s) - fluorophore , intramolecular force , fluorescence , chemistry , molecule , photoinduced electron transfer , biophysics , electron transfer , photochemistry , stereochemistry , optics , biology , physics , organic chemistry
Triggered by ions and antibodies : Fluorescent sensors based on intramolecular photoinduced electron transfer (PET) enable the monitoring of individual recognition and binding events at the single‐molecule level with high optical and temporal resolution. The fluorophore can be directly involved in the binding process or act as reporter sensitive to binding‐induced conformational changes, for example, conformational changes within a peptide induced by antibody binding (see scheme).