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Scission of DNA with Bis(1,10‐phenanthroline)copper without Intramolecular Hydrogen Migration
Author(s) -
Zelenko Ottilie,
Gallagher James,
Sigman David S.
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199727761
Subject(s) - bond cleavage , intramolecular force , chemistry , cleavage (geology) , nuclease , dna , phenanthroline , copper , thymidine , stereochemistry , crystallography , biochemistry , biology , catalysis , organic chemistry , fracture (geology) , paleontology
C‐1′H and not C‐2′H is the initial site of oxidative cleavage of DNA with the chemical nuclease [Cu(phen) 2 ] + /H 2 O 2 . This is the result of experiments with DNA containing C‐1′‐deuterated thymidine groups (the first step of the reaction sequence is shown below). If C‐2′H is extracted in the first step, a one‐electron oxidation and H/D migration should take place; there is no evidence of this.