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Substrate Mimetic Mediated Peptide Synthesis: An Irreversible Ligation Strategy That Is Independent of Substrate Specificity
Author(s) -
Bordusa Frank,
Ullmann Dirk,
Elsner Christian,
Jakubke HansDieter
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199724731
Subject(s) - peptide , chemistry , substrate (aquarium) , trypsin , protease , proteases , biochemistry , dna ligase , peptide bond , substrate specificity , ligation , thrombin , enzyme , biology , microbiology and biotechnology , ecology , platelet , immunology
Cationic specificity determinants of substrate mimetics 1 bind optimally to the binding site of highly specific proteases that normally determines their primary specificity. This designed imitation not only allows trypsin‐catalyzed nonspecific and irreversible peptide‐bond formation, but now also the use of thrombin and especially the cystein protease clostripain, which acts as the most efficient peptide ligase.