Efficient Enzymatic Synthesis of the Core Trisaccharide of  N ‐Glycans with a Recombinant β‐Mannosyltransferase | Zendy

Watt Gregory M. | Zendy; Revers Leigh | Zendy; Webberley Matthew C. | Zendy; Wilson Iain B. H. | Zendy; Flitsch Sabine L. | Zendy

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Efficient Enzymatic Synthesis of the Core Trisaccharide of N ‐Glycans with a Recombinant β‐Mannosyltransferase

Author(s) -

Watt Gregory M.,

Revers Leigh,

Webberley Matthew C.,

Wilson Iain B. H.,

Flitsch Sabine L.

Publication year - 1997

Publication title -

angewandte chemie international edition in english

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 0570-0833

DOI - 10.1002/anie.199723541

Subject(s) - trisaccharide , recombinant dna , chemistry , glycan , regioselectivity , enzyme , yeast , biocatalysis , saccharomyces cerevisiae , stereochemistry , biochemistry , combinatorial chemistry , glycoprotein , catalysis , gene , ionic liquid

A highly stereo‐ and regioselective linkage for the β‐1,4‐mannoside bond of trisaccharide 1 (R/R′ = H/OH) is achieved with a redesigned recombinant biocatalyst: a suitably modified yeast enzyme (ALG1) was expressed in E. coli ; the enzyme was then immobilized on a Ni II affinity column.

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