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Efficient Enzymatic Synthesis of the Core Trisaccharide of N ‐Glycans with a Recombinant β‐Mannosyltransferase
Author(s) -
Watt Gregory M.,
Revers Leigh,
Webberley Matthew C.,
Wilson Iain B. H.,
Flitsch Sabine L.
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199723541
Subject(s) - trisaccharide , recombinant dna , chemistry , glycan , regioselectivity , enzyme , yeast , biocatalysis , saccharomyces cerevisiae , stereochemistry , biochemistry , combinatorial chemistry , glycoprotein , catalysis , gene , ionic liquid
A highly stereo‐ and regioselective linkage for the β‐1,4‐mannoside bond of trisaccharide 1 (R/R′ = H/OH) is achieved with a redesigned recombinant biocatalyst: a suitably modified yeast enzyme (ALG1) was expressed in E. coli ; the enzyme was then immobilized on a Ni II affinity column.