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Site‐Specific Photocleavage of Proteins
Author(s) -
Kumar Challa V.,
Buranaprapuk Apinya
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199720851
Subject(s) - cleavage (geology) , conjugate , chemistry , pyrene , bovine serum albumin , phenylalanine , fluorescence , stereochemistry , biochemistry , biology , amino acid , organic chemistry , optics , mathematical analysis , paleontology , physics , mathematics , fracture (geology)
Bovine serum albumin and chicken‐egg lsoyzyme can be cleaved with high specificity into two fragments by the method shown below. A pyrene‐ L ‐phenylalanine conjugate was used as the probe (shown schematically over the first arrow), which binds with site specificity to the protein and produces the cleavage upon irradiation with visible light in the presence of a Co III compound.