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NADH‐Dependent Halogenases Are More Likely To Be Involved in Halometaolite Biosynthesis Than Haloperoxidases
Author(s) -
Hohaus Kathrin,
Altmann Annett,
Burd Wassily,
Fischer Ilona,
Hammer Philip E.,
Hill Dwight S.,
Ligon James M.,
van Pée KarlHeinz
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199720121
Subject(s) - pseudomonas fluorescens , tryptophan , biochemistry , regioselectivity , biosynthesis , chemistry , substrate (aquarium) , substrate specificity , enzyme , peroxide , pseudomonas , stereochemistry , organic chemistry , biology , bacteria , catalysis , amino acid , genetics , ecology
NADH instead of hydroged peroxide is required by two newly identified, substrate‐specific and regioselective halogenases. Both were isolated from a pyrrolnitrin‐producing Pseudomonas fluorescens strain. One enzyme catalyzes the chlorination of L ‐tryptophan to 7‐chloro‐ L ‐tryptophan ( 1 ).