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In Vitro Selection without Intervening Amplification
Author(s) -
Smith Joseph,
Anslyn Eric V.
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199718791
Subject(s) - selection (genetic algorithm) , aptamer , in vitro , oligonucleotide , dna , computer science , microbiology and biotechnology , computational biology , polymerase , chemistry , biology , biochemistry , artificial intelligence
Iterative screenings without enzymatic amplification were successfully performed for DNA aptamers to ATP, thus demonstrating that in vitro selection of oligonucleotide libraries is possible without a polymerase step. The approach is shown schematically on the right.