Premium
A Straightforward Solid‐Phase Synthesis of Cyclic Oligodeoxyribonucleotides
Author(s) -
Alazzouzi ElMostafa,
Escaja Nuria,
Grandas Anna,
Pedroso Enrique
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199715061
Subject(s) - solid phase synthesis , combinatorial chemistry , cleavage (geology) , oligonucleotide , chemistry , polymer , impurity , phase (matter) , materials science , organic chemistry , biochemistry , dna , peptide , fracture (geology) , composite material
For possible use as antisense or antigene therapeutic agents , fairly pure crude cyclic oligonucleotides can easily be prepared by solid‐phase synthesis (see below). After cyclization with formation of a phosphotriester (a) and cleavage (b), all noncyclized compounds, polymers, and other impurities are removed by filtration (c).