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Fluorescence Reporters for Phosphodiesterase Activity
Author(s) -
Berkessel Albrecht,
Riedl Rainer
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199714811
Subject(s) - fluorescence , fluorophore , chemistry , quenching (fluorescence) , phosphodiesterase , intramolecular force , photochemistry , biophysics , nuclease , hydrolysis , biochemistry , enzyme , biology , stereochemistry , optics , physics
A sensitive indicator of nuclease activity is the fluorescence behavior of phosphodiesters 1 and 2 . Although 1 and 2 do not fluoresce due to intramolecular quenching, the strongly fluorescent naphthyl phosphates and naphthols are liberated upon hydrolysis. This gives rise to an extremely strong increase in fluorescence intensity. Combined application of 1 and 2 allows detection of phosphatase in addition to phosphodiesterase activity. f = fluorophore, q = quencher.