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Protease‐Catalyzed Peptide Synthesis from N‐ to C‐Terminus: An Advantageous Strategy
Author(s) -
Bordusa Frank,
Ullmann Dirk,
Jakubke HansDieter
Publication year - 1997
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199710991
Subject(s) - proteases , stereocenter , protease , peptide , catalysis , enzyme , chemistry , hydrolysis , biochemistry , combinatorial chemistry , stereochemistry , enantioselective synthesis
Not possible with chemical methods: Protein synthesis from the N‐ to C‐ terminus using the reverse hydrolysis potential of proteases was accompolished for the model petide 1 . The stereo‐ and regiospecificity of the enzymes used ensured integrity of the stereogenic center and allowed ecological reaction conditions without side‐chain protection.