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Enhancing DNA Triple Helix Stability at Neutral pH by the Use of Oligonucleotides Containing a More Basic Deoxycytidine Analog
Author(s) -
Hildbrand Stefan,
Leumann Christian
Publication year - 1996
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199619681
Subject(s) - oligonucleotide , triple helix , dna , chemistry , deoxycytidine , protonation , base pair , nucleoside , stereochemistry , nucleotide , nucleobase , combinatorial chemistry , crystallography , biophysics , biochemistry , biology , organic chemistry , genetics , gene , ion , chemotherapy , gemcitabine
DNA triple helices in the parallel binding motif can efficiently be stabilized at neutral pH by replacing deoxycytidine in the third strand by the C‐nucleoside 1 . The increase in stability is due to the higher p K a of the base in 1 , rendering its protonation, required for base pair formation, more favorable at physiological pH.