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The First Recombinant Hydroxynitrile Lyase and its Application in the Synthesis of ( S )‐Cyanohydrins
Author(s) -
Förster Siegfried,
Roos Jürgen,
Effenberger Franz,
Wajant Harald,
Sprauer Achim
Publication year - 1996
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199604371
Subject(s) - enantioselective synthesis , chemistry , alkyl , recombinant dna , cyanohydrin , aryl , manihot esculenta , lyase , organic chemistry , stereochemistry , catalysis , combinatorial chemistry , enzyme , biochemistry , botany , biology , gene
The cassava species Manihot esculenta provided the first recombinant hydroxynitrile lyase (MeHNL) that became accessible by overexpression in E. coli. This MeHNL catalyzes the enantioselective addition of HCN (depicted below) not only to aliphatic, aromatic, and heteroaromatic aldehydes, but also to ketones to give ( S )‐cyanohydrins in high optical yields. R alkyl, aryl, heteroaryl; R 1 H, CH 3 .