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Vitamin B 12 : The Haze Clears
Author(s) -
Kräutler Bernhard,
Kratky Christoph
Publication year - 1996
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199601671
Subject(s) - methylcobalamin , vitamin b12 , methionine synthase , chemistry , cofactor , histidine , residue (chemistry) , stereochemistry , cyanocobalamin , cobalt , methionine , biochemistry , amino acid , organic chemistry , enzyme
The first X‐ray analysis of a B 12 protein , the B 12 ‐binding domain of the methionine synthase of Escherichia coli , led to a surprising finding: there the B 12 derivative, methylcobalamin, is not bound in the “base‐on” form present in solution and in the crystal. Instead a histidine residue coordinates at the cobalt center of the protein‐bound cofactor in place of the nucleotide function.