Premium
Synthesis of dTDP‐6‐Deoxy‐4‐ketoglucose and Analogues with Native and Recombinant dTDP‐Glucose‐4,6‐dehydratase
Author(s) -
Stein Andreas,
Kula MariaRegina,
Elling Lothar,
Verseck Stefan,
Klaffke Werner
Publication year - 1995
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199517481
Subject(s) - dehydratase , deoxygenation , biochemistry , enzyme , chemistry , recombinant dna , catalysis , gene
The enzymatic deoxygenation of dTDP‐glucose and two 3‐deoxy analogues [Eq. (a)] is now possible on a preparative scale with dTDP‐ D ‐glucose‐4,6‐dehydratase. In addition a highly selective HPLC method for the separation of structurally related activated sugars has been developed. This should provide new impetus for structure–function studies of deoxysugars and further elucidation of their biosynthetic pathways. R OH, H, N 3 ; dTDP = thymidine‐5′‐diphosphate.