Premium
Photochemically Initiated Protein Splicing
Author(s) -
Cook Sandra N.,
Jack William E.,
Xiong Xiaofeng,
Danley Lora E.,
Ellman Jonathan A.,
Schultz Peter G.,
Noren Christopher J.
Publication year - 1995
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199516291
Subject(s) - rna splicing , protein splicing , chemistry , alternative splicing , thermococcus , splicing factor , microbiology and biotechnology , biochemistry , biology , gene , messenger rna , rna , archaea
More details on the mechanism of protein splicing were revealed from the substitution of O ‐(2‐nitrobenzyl)serine for the conserved Ser1082 at the upstream splice junction of the self‐splicing DNA polymerase of Thermococcus litoralis . Full length precursor protein was produced, which underwent protein splicing only upon photolysis.