Premium
Chemoenzymatic Synthesis of a Modified Pentasaccharide as a Specific Substrate for a Sensitive Assay of α‐Amylase by Fluorescence Quenching
Author(s) -
Payre Nathalie,
Cottaz Sylvain,
Driguez Hugues
Publication year - 1995
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199512391
Subject(s) - amylase , chemistry , fluorescence , quenching (fluorescence) , fluorophore , substrate (aquarium) , enzyme , hydrolysis , cellulase , biochemistry , chromatography , biology , ecology , physics , quantum mechanics
Resonance energy transfer (RET) is exploited in a novel assay of endoglucanase activity (see scheme below). The variation in the fluorescence intensity of a fluorogenic oligosaccharide is followed during its hydrolysis, allowing the determination of α‐amylase activity, one of the starch‐degrading enzymes, which is important for diagnosing diseases like parotitis (mumps) and acute pancreatitis.= sugar unit; F 1 ,F 2 = fluorophore.