Chemoenzymatic Synthesis of a Modified Pentasaccharide as a Specific Substrate for a Sensitive Assay of α‐Amylase by Fluorescence Quenching | Zendy

Payre Nathalie | Zendy; Cottaz Sylvain | Zendy; Driguez Hugues | Zendy

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Chemoenzymatic Synthesis of a Modified Pentasaccharide as a Specific Substrate for a Sensitive Assay of α‐Amylase by Fluorescence Quenching

Author(s) -

Payre Nathalie,

Cottaz Sylvain,

Driguez Hugues

Publication year - 1995

Publication title -

angewandte chemie international edition in english

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 0570-0833

DOI - 10.1002/anie.199512391

Subject(s) - amylase , chemistry , fluorescence , quenching (fluorescence) , fluorophore , substrate (aquarium) , enzyme , hydrolysis , cellulase , biochemistry , chromatography , biology , ecology , physics , quantum mechanics

Resonance energy transfer (RET) is exploited in a novel assay of endoglucanase activity (see scheme below). The variation in the fluorescence intensity of a fluorogenic oligosaccharide is followed during its hydrolysis, allowing the determination of α‐amylase activity, one of the starch‐degrading enzymes, which is important for diagnosing diseases like parotitis (mumps) and acute pancreatitis.= sugar unit; F 1 ,F 2 = fluorophore.

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