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Modified Triple Resonance NMR Experiments with Optimized Sensitivity for Rapidly Exchanging Protons
Author(s) -
Jahnke Wolfgang,
Kessler Horst
Publication year - 1995
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199504691
Subject(s) - pulse sequence , amide , nuclear magnetic resonance , sensitivity (control systems) , chemistry , resonance (particle physics) , residue (chemistry) , pulse (music) , analytical chemistry (journal) , materials science , physics , atomic physics , chromatography , organic chemistry , optics , electronic engineering , detector , engineering
Fast exchange with surrounding water protons need not attenuate 1 H NMR signals of proteins in solution. A modified pulse sequence has been developed to increase the signal intensity of the amide protons; on the right the improvement in sensitivity (a → c) is shown for a selected residue in a protein. This new technique should aid elucidation of protein structures in solution.