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Micron‐Scale Patterning of Biological Molecules
Author(s) -
Pritchard David John,
Morgan Hywel,
Cooper Jonathan Mark
Publication year - 1995
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199500911
Subject(s) - analyte , nanotechnology , avidin , materials science , molecule , silicon dioxide , silicon , ligand (biochemistry) , chemistry , receptor , optoelectronics , biotin , chromatography , biochemistry , organic chemistry , composite material
Site‐specific attachment of biological molecules on gold and silicon dioxide surfaces has been achieved: The surface is modified with the receptor/ligand pair avidin D/photobiotin 1 and exposed to light through a mask in the presence of a protein solution; binding only occurs in the exposed areas. After unbound material is removed by washing, this process can be repeated several times to produce a two‐dimensional pattern. One of the potential applications of this technique is the patterning of antibodies in a format that can be used in a multi‐analyte immunosensor.