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Keronopsins A and B, Chemical Defence Substances of the Marine Ciliate Pseudokeronopsis rubra (Protozoa): Identification by Ex Vivo HPLC
Author(s) -
Höfle Gerhard,
Pohlan Silke,
Uhlig Gotram,
Kabbe Karin,
Schumacher Dorothee
Publication year - 1994
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199414951
Subject(s) - ex vivo , high performance liquid chromatography , protozoa , chemistry , chromatography , in vivo , biochemistry , biology , botany , in vitro , microbiology and biotechnology
What happens when protozoa are injected onto an HPLC column? Because they do not have a rigid cell wall, they instantaneously release their contents, which can thus be analyzed without loss or opportunity for artefact formation (“ex vivo HPLC”). This method applied to the example, Pseudokeronopsis rubra (picture below), showed that the organism stores 1 in the form of the sulfate ester (R 1 = SO 3 Na), which only on destruction of the cells is converted enzymatically into the more toxic pyrone 1 (R 1 = H), R 2 = H, Br.