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Recombinant Whole Cells as Catalysts for the Enzymatic Synthesis of Oligosaccharides and Glycopeptides
Author(s) -
Herrmann Guido F.,
Wang Peng,
Shen GwoJenn,
Wong ChiHuey
Publication year - 1994
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199412411
Subject(s) - periplasmic space , glycopeptide , monosaccharide , recombinant dna , enzyme , biochemistry , chemistry , glycan , escherichia coli , glycosaminoglycan , glycoprotein , antibiotics , gene
An easier, faster, cheaper approach to the enzymatic synthesis of oligosaccharides would be to employ whole cells rather than purified enzymes. In this report recombinant E. coli cells mediate the mannosylation of monosaccharides and glycopeptides. It was also proven that the relevant enzyme, mannosyl transferase, does not leak from the periplasmic space of the cells into the medium.