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An Amazing Distortion in DNA Induced by a Methyltransferase (Nobel Lecture)
Author(s) -
Roberts Richard J.
Publication year - 1994
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199412221
Subject(s) - dna , helix (gastropod) , dna methyltransferase , base pair , biophysics , methyltransferase , chemistry , crystallography , biology , biochemistry , methylation , ecology , snail
Not a bend or kink in the DNA , but the flip of the base to be methylated out the DNA helix (picture on the right) occurs when a methyltransferase binds to DNA. This was the key result of the X‐ray structure analysis of a ternary complex of M. Hha I, AdoMet, and a 5‐fluorocytosine‐substituted DNA double helix. This conformational shift may generally play a major role in the opening up of DNA helices.