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Isolation of RNA Aptamers for Biological Cofactors by In Vitro Selection
Author(s) -
Burgstaller Petra,
Famulok Michael
Publication year - 1994
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199410841
Subject(s) - flavin mononucleotide , cofactor , flavin adenine dinucleotide , rna , flavin group , biochemistry , ribozyme , agarose , chemistry , biology , stereochemistry , enzyme , gene
Short RNA sequences with specific secondary structure (see below) are the binding motifs for the cofactors flavin adenine dinucleotide and flavin mononucleotide (FAD and FMN, respectively), according to selection experiments with randomized RNA pools and FAD‐ and FMN‐modified agarose. It was also shown that only the flavin portion plays a role in binding. Analogous experiments were conducted with NAD + and its mononucleotide.