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Progress toward an Antibody Glycosidase
Author(s) -
Yu Jaehoon,
Hsieh Linda C.,
Kochersperger Lynn,
Yonkovich Shirlee,
Stephans James C.,
Gallop Mark A.,
Schultz Peter G.
Publication year - 1994
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199403391
Subject(s) - hapten , chemistry , hydrolysis , glycoside hydrolase , antibody , monoclonal antibody , substrate (aquarium) , piperidine , stereochemistry , biochemistry , enzyme , combinatorial chemistry , biology , immunology , ecology
The piperidine‐based hapten 1 generates monoclonal antibodies that catalyze the hydrolysis of substrate 2 at pH5.5 ( k cal = 0.904 h −1 , K M = 324 μM). Hapten 1 is a competitive inhibitor of the antibody‐catalyzed reaction. These results are significant for the development of antibodies for the sequence‐specific hydrolysis of oligosaccharides.