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Ion‐Spray Mass Spectrometry and High‐Performance Liquid Chromatography—Mass Spectrometry of Synthetic Peptide Libraries
Author(s) -
Metzger Jörg W.,
Wiesmüller KarlHeinz,
Gnau Volker,
Brünjes Jente,
Jung Günther
Publication year - 1993
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199308941
Subject(s) - mass spectrometry , peptide , chemistry , chromatography , major histocompatibility complex , high performance liquid chromatography , sample preparation in mass spectrometry , protein mass spectrometry , peptide fragment , tandem mass spectrometry , combinatorial chemistry , biochemistry , electrospray ionization , gene
Optimizing syntheses and characterizing complex mixtures of peptides (peptide libraries)—crucial to the search for new lead structures in drug development—is possible with ion‐spray mass spectrometry alone or combined with high‐performance liquid chromatography. This was shown in investigations of a small sublibrary made up of 48 octapeptides, which represents 1 % of the peptide pool exhibiting the murine major histocompatibility complex (MHC) class I H2‐K b restricted sequence motif, and studies of a mixture of 24 576 peptides that bind to murine MHC class I Qa‐2 a molecules.