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Capillary Electrophoresis: Methods and Scope
Author(s) -
Engelhardt Heinz,
Beck Wolfgang,
Kohr Jörg,
Schmitt Thomas
Publication year - 1993
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199306293
Subject(s) - capillary electrophoresis , micellar electrokinetic chromatography , chromatography , electrophoresis , chemistry , electrokinetic phenomena , enantiomer , free flow electrophoresis , isotachophoresis , micelle , scope (computer science) , affinity electrophoresis , gel electrophoresis , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , computer science , affinity chromatography , organic chemistry , biochemistry , aqueous solution , electrode , electrolyte , enzyme , programming language
Capillary electrophoresis is a new analytical technique that has seen a great upswing in recent years, because this adaptation of electrophoresis may be automated and simple, direct quantification is possible. As in conventional electrophoresis, samples with a large range of molecular weights, from inorganic ions to biopolymers such as DNA and proteins, can be separated. Uncharged molecules can also be separated when micelle‐forming detergents are added to the buffer; the distribution mechanism that then comes into play increases the separation efficiency of this micellar electrokinetic chromatography. Chiral additives such as cyclodextrins make the separation of enantiomers possible. The rapid increase in the popularity of capillary electrophoresis is reflected in instrument sales and the growing number of scientific publications dealing with the method, and it seems that the future of this technique is assured.