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Selective Photolabeling near the Middle of Bilayers with a Photosensitive Transmembrane Probe
Author(s) -
Yamamoto Masakuni,
Warnock William A.,
Milon Alain,
Nakatani Yoichi,
Ourisson Guy
Publication year - 1993
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199302591
Subject(s) - vesicle , phospholipid , chemistry , membrane , transmembrane protein , selectivity , biophysics , lipid bilayer , photochemistry , biochemistry , biology , receptor , catalysis
The efficient study of the inside of a membrane should no longer be a problem, thanks to the investigations described here. In vesicles from phospholipids, incorporated 1 (X H,D) reacts upon irradiation with a selectivity up to 90% at positions ω −1 and ω −2 of the phospholipid chains, when, at the same time, cholesterol is a constituent of the membrane in normal bioconcentrations.