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Detection of Subpicomole Levels of Compounds Containing Hydroxyl and Amino Groups with the Fluorogenic Reagent, 2‐Naphthoylimidazole
Author(s) -
Ikemoto Norihiro,
Lo LeeChiang,
Nakanishi Koji
Publication year - 1992
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199208901
Subject(s) - reagent , chemistry , yield (engineering) , chromatography , fluorescence , sugar , high performance liquid chromatography , derivatization , organic chemistry , materials science , physics , quantum mechanics , metallurgy
Even one tenth of a picomole of a sugar can be detected, if it is transformed into the alditol and treated with 1 to yield the pernaphthoate. In this way a mixture of nine sugars and aminosugars could be well separated by HPLC. The pernaphthoates were detected by their intense fluorescence at 374 nm, thus proving that 1 is ideal for derivatizing natural products containing hydroxyl and amino groups for analytical purposes.