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Chemical Attachment of Organometallics to Proteins in Reverse Micelles
Author(s) -
Ryabov Alexander D.,
Trushkin Alexey M.,
Baksheeva Lidia I.,
Gorbatova Raisa K.,
Kubrakova Irina V.,
Mozhaev Vadim V.,
Gnedenko Boris B.,
Levashov Andrey V.
Publication year - 1992
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199207891
Subject(s) - chemistry , micelle , covalent bond , acetonitrile , microemulsion , residue (chemistry) , glucose oxidase , microreactor , boron , organic chemistry , combinatorial chemistry , octane , polymer chemistry , enzyme , catalysis , biochemistry , pulmonary surfactant , aqueous solution
For the construction of new biocatalysts it might be necessary to attach a water‐insoluble organometallic residue covalently to a soluble enzyme. Reverse micelles—“microreactors” with a polar center and a nonpolar shell—can achieve this, as shown by the covalent binding of carboxyferrocene (FcCOOH) to glucose oxidase (ENH 2 ) in the presence of dicyclohexylcarbodiimide/ N ‐hydroxysuccinimide as activating couple [Eq. (a)] in a system containing borate buffer, n ‐octane, aerosol OT, and acetonitrile.
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