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Photolithographic Immobilization of Biopolymers on Solid Supports
Author(s) -
Rozsnyai Lawrence F.,
Benson David R.,
Fodor Stephen P. A.,
Schultz Peter G.
Publication year - 1992
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199207591
Subject(s) - biomolecule , covalent bond , biosensor , benzophenone , chemistry , ligand (biochemistry) , fluorescence , fluorescence microscope , molecule , nanotechnology , opacity , biophysics , materials science , polymer chemistry , biochemistry , optics , receptor , organic chemistry , biology , physics
The new technique of site‐specific immobilization of biomolecules , for example antibodies, may contribute to the development of biosensors and ligand‐binding assays. (The key step is shown schematically here.) A benzophenone‐derivatized surface is treated with a solution of the antibody (Ig) and then irradiated through a mask with a checkerboard pattern of transparent and opaque fields. The Ig molecules are bound covalently only in areas which were exposed to light. This was proved by epifluorescence microscopy after the surface was washed free of unbound Ig.