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Radical Intermediates in the Coenzyme B 12 Dependent Methylmalonyl‐CoA Mutase Reaction Shown by ESR Spectroscopy
Author(s) -
Zhao Ymin,
Such Peter,
Rétey János
Publication year - 1992
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199202151
Subject(s) - mutase , chemistry , cofactor , substrate (aquarium) , enzyme , spectroscopy , stereochemistry , biochemistry , biology , physics , ecology , quantum mechanics
Only after the addition of the substrate, methylmalonyl‐CoA , to the binary complex of highly purified methylmalonyl‐CoA mutase and coenzyme B 12 is a clear ESR signal observed. This proves that radical intermediates that have only been postulated in the past are indeed involved in the enzymatic rearrangement of the carbon skeleton.