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Antibody Catalysis of Glycosidic Bond Hydrolysis
Author(s) -
Reymond JeanLouis,
Janda Kim D.,
Lerner Richard A.
Publication year - 1991
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199117111
Subject(s) - hapten , glycosidic bond , chemistry , hydrolysis , substituent , antibody , catalysis , stereochemistry , leaving group , biochemistry , organic chemistry , enzyme , immunology , biology
For the preparation of abzymes with glycolytic activity , the generation of an antibody that catalyzes the hydrolysis of acetal 1 represents a first step. The antibody (or abzyme) is obtained from immunization with piperidinium hapten 2. A key factor in promoting catalysis is probably the proper placement of the leaving group in 1 as axial substituent.