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A New Chromogenic β‐Galactosidase Substrate: 7‐β‐ D ‐Galactopyranosyloxy‐9,9‐dimethyl‐9 H ‐acridin‐2‐one
Author(s) -
Corey Paul F.,
Trimmer Robert W.,
Biddlecom William G.
Publication year - 1991
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199116461
Subject(s) - chromogenic , substrate (aquarium) , hydrolysis , chemistry , enzyme , combinatorial chemistry , biochemistry , chromatography , biology , ecology
Hydrolysis of the title compound at pH 8 causes a color shift of 196 nm (438 → 634 nm). The total synthesis, kinetic parameters, and application in enzyme immunoassays are described.