Premium
Direct Observation of Streptavidin Specifically Adsorbed on Biotin‐Functionalized Self‐Assembled Monolayers with the Scanning Tunneling Microscope
Author(s) -
Häussling Lukas,
Michel Bruno,
Ringsdorf Helmut,
Rohrer Heinrich
Publication year - 1991
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199105691
Subject(s) - monolayer , scanning tunneling microscope , streptavidin , adsorption , scanning electron microscope , adhesion , biotin , self assembled monolayer , nanotechnology , self assembly , materials science , crystallography , chemistry , organic chemistry , composite material , biochemistry
Surface recognition of biotin‐functionalized, chemisorbed thiol monolayers by streptavadin results in the formation of protein‐covered surfaces with no longrange order. This is due to the low lateral and vertical mobility of the solidbound thiols. On the other hand, protein surfaces transferred from a water surface reveal a good crystal structure under the scanning electron microscope, but the adhesion of these applied layers to the solid is unsatisfactory.