Isolation of an  L ‐Stereospecific  N ‐Acyl‐ L ‐proline‐Acylase and its Use as Catalyst in Organic Synthesis | Zendy

Groeger Ulrich | Zendy; Drauz Karlheinz | Zendy; Klenk Herbert | Zendy

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Isolation of an L ‐Stereospecific N ‐Acyl‐ L ‐proline‐Acylase and its Use as Catalyst in Organic Synthesis

Author(s) -

Groeger Ulrich,

Drauz Karlheinz,

Klenk Herbert

Publication year - 1990

Publication title -

angewandte chemie international edition in english

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 0570-0833

DOI - 10.1002/anie.199004171

Subject(s) - stereospecificity , proline , comamonas testosteroni , chemistry , enzyme , hydrolysis , bacteria , stereochemistry , catalysis , biochemistry , organic chemistry , amino acid , biology , genetics

Microbiological screening has resulted in the selection of the microorganism Comamonas testosteroni . Growth of this bacterium in the presence of N ‐acetyl‐ L ‐proline leads to formation of the enzyme N ‐acyl‐ L ‐proline acylase. The enzyme (molecular weight 380 ± 40 kDa, with eight identical subunits),is thermally stable to 70 °C. At pH values between 5 and 10 the enzyme causes stereospecific hydrolysis of N ‐acetyl‐ L ‐proline, making it useful for the resolution of racemic D,L ‐proline mixtures.

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