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Continuous Generation of NADH from NAD ⊕ and Formate Using a Homogeneous Catalyst with Enhanced Molecular Weight in a Membrane Reactor
Author(s) -
Steckhan Eberhard,
Herrmann Sabine,
Ruppert Romain,
Thömmes Jörg,
Wandrey Christian
Publication year - 1990
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.199003881
Subject(s) - nad+ kinase , formate , catalysis , yield (engineering) , membrane , membrane reactor , formate dehydrogenase , chemistry , cofactor , redox , polyethylene glycol , reactivity (psychology) , materials science , enzyme , organic chemistry , biochemistry , medicine , alternative medicine , pathology , metallurgy
Bonding the Rh III complex 1 to polyethylene glycol (MW 20 000) leads to a high molecular weight redox catalyst suitable for continuous generation of the cofactors NADH and NADPH. With the aid of a newly designed flow‐through membrane reactor it has proven possible to achieve an NAD ⊕ conversion of 40–45% (turnover number 16.9 h −1 , space‐time yield 44.3mmol L −1 d −1 ). Since the reactivity towards polyethylene glycol‐bound NAD ⊕ remains constant, this catalyst is also suited to use in a continuously operating enzyme‐membrane reactor.