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Laser Desorption/Ionization Mass Spectrometry of Proteins of Mass 100 000 to 250 000 Dalton
Author(s) -
Karas Michael,
Bahr Ute,
Ingendoh Arndt,
Hillenkamp Franz
Publication year - 1989
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198907601
Subject(s) - mass spectrometry , chemistry , molecular mass , ion , desorption , matrix assisted laser desorption/ionization , ionization , laser , analytical chemistry (journal) , surface enhanced laser desorption/ionization , polyatomic ion , irradiation , chromatography , oligomer , sample preparation in mass spectrometry , adsorption , electrospray ionization , biochemistry , enzyme , optics , organic chemistry , physics , nuclear physics
Irradiation of proteins in a nicotinic acid matrix with the 266‐nm light of a Nd‐YAG laser allowed the first determination of molecular masses up to 250 000 daltons. Since the peaks of multiply charged molecular ions and of oligomer ions can also be used in determination of molecular mass, this method attains a precision of ca. 0.1%. Its potential is demonstrated for β‐ D ‐galactosidase, glucose isomerase, and catalase.