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Biosynthetic Studies on Antibiotics
Author(s) -
Floss Heinz G.,
Beale John M.
Publication year - 1989
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198901461
Subject(s) - chemistry , stereospecificity , isotopic labeling , biosynthesis , cloning (programming) , computational biology , combinatorial chemistry , stereochemistry , biochemistry , gene , biology , organic chemistry , computer science , programming language , catalysis
The intricacies of the synthetic chemistry executed by living cells in the assembly of complex organic natural products can be probed in great detail by stable‐isotope labeling in conjunction with sophisticated 2D and multiple‐quantum NMR methods of analysis. Mechanistic details are revealed by unraveling the “cryptic” stereochemistry of biosynthetic reactions with the aid of stereospecific isotope labeling. Analysis of blocked mutants and cloning of biosynthetic genes provides new tools for the study of biosynthetic pathways and for the exploitation of the chemical capabilities of living organisms.