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NOESY‐TOCSY, an Advantageous 2D NMR Technique for the Analysis of Peptide Sequences
Author(s) -
Kessler Horst,
Gemmecker Gerd,
Steuernagel Stefan
Publication year - 1988
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198805641
Subject(s) - peptide , two dimensional nuclear magnetic resonance spectroscopy , scalar (mathematics) , molecule , peptide bond , nuclear magnetic resonance spectroscopy , chemistry , relaxation (psychology) , spectral line , sequence (biology) , biological system , physics , nuclear magnetic resonance , mathematics , biology , organic chemistry , quantum mechanics , biochemistry , geometry , neuroscience
Molecules that exhibit strong negative NOEs and line broadening in the NMR spectrum due to short relaxation times are particularly good objects of investigations using the variants of unsymmetric 2D NMR techniques discussed here. Undesired signals can be suppressed by data processing, thus allowing, for example, the amino acid sequence of a peptide or protein to be easily determined. The nonsymmetry results from the fact that, across the peptide bond, NOE but no scalar coupling of protons is observed.