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Structure Elucidation of a New Icosapeptide from Human Semen
Author(s) -
Schneider Klaus,
Reiner Josef,
Spiteller Gerhard
Publication year - 1987
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198707741
Subject(s) - edman degradation , chromatography , mass spectrometry , chemistry , degradation (telecommunications) , sequence (biology) , peptide sequence , biochemistry , engineering , telecommunications , gene
Only 300 μg of the new oligopeptide 1 was available for a structure determination. The sequence could be determined by a combination of liquid secondary ion mass spectrometry (LSIMS) and Edman degradation. Crucial for this success was the observation that the sample used for the mass spectrometric analysis could be eluted unchanged from the target and subsequently used for an Edman degradation.