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First ENDOR Investigations of Biologically Relevant Organic Radicals in Reversed Micelles
Author(s) -
Bretz Norbert,
Mastalsky Ingrid,
Elsner Martin,
Kurreck Harry
Publication year - 1987
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198703451
Subject(s) - flavin mononucleotide , chemistry , flavin group , flavin adenine dinucleotide , micelle , radical , cofactor , aqueous solution , photochemistry , flavoprotein , electron paramagnetic resonance , solvent , combinatorial chemistry , organic chemistry , enzyme , nuclear magnetic resonance , physics
Reversed micelles as problem solvers : They stabilize enzymes with respect to hydrophobic interactions, suppress possibly undesired properties of modified cofactors such as aggregate formation, and now make possible, among other things, EPR and ENDOR spectroscopy of the radical anions of riboflavin, flavin mononucleotide (FMN), and flavin adenine dinucleotide (FAD) in aqueous media, without water being the actual solvent. In this way, problems arising from high microwave dielectric loss can be avoided.