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15 N‐NMR Spectroscopy—New Methods and Applications [New Analytical Methods (28)]
Author(s) -
von Philipsborn Wolfgang,
Müller Raffaello
Publication year - 1986
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198603833
Subject(s) - nuclear magnetic resonance spectroscopy , chemistry , spectroscopy , molecule , fluorine 19 nmr , organic molecules , chemical physics , analytical chemistry (journal) , organic chemistry , physics , quantum mechanics
The nitrogen nucleus is the third most important probe (after 1 H and 13 C) for structural investigations of organic and bioorganic molecules by NMR spectroscopy. For a long time, however, the insufficient sensitivity and low natural abundance of the 15 N isotope hampered detection of the 15 N nucleus, and the quadrupolar 14 N nucleus proved unsuitable for the study of larger molecules with several nonequivalent nitrogen atoms. The advent of new techniques, such as pulse sequences and polarization transfer, in conjunction with the use of high‐field magnets and large‐sample probe heads largely solved the detection problem. As a result, the last few years have seen a dramatic development of 15 N‐NMR spectroscopy as a versatile method for studying molecular structure, both in isotropic (liquid) and anisotropic (solid) phases. The scope of chemical applications extends from inorganic, organometallic, and organic chemistry to biochemistry and molecular biology, and includes the study of reactive intermediates, biopolymers, enzyme‐inhibitor complexes, and nitrogen metabolism. Two‐dimensional NMR techniques offer additional possibilities for detailed studies of biological systems.