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Three‐Phase Synthesis of Oligonucleotides
Author(s) -
Seliger Hartmut,
Gupta Kailash Chand
Publication year - 1985
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198506851
Subject(s) - phosphoramidite , oligonucleotide , reagent , tetrazole , oligonucleotide synthesis , nucleoside , solid phase synthesis , combinatorial chemistry , chemistry , nucleic acid , organic chemistry , stereochemistry , dna , biochemistry , peptide
Polymer‐bound reagents of the nucleoside phosphoramidite type , such as 1 (P = Merrifield resin, B = thymin‐1‐yl, DMTr = dimethoxytrityl) are superbly suitable for the chemical synthesis of oligonucleotides. Upon activation with tetrazole, they are released and can be coupled with polymerbound nucleosides. 1 is stable upon storage and reacts after 15 months with the same yields.