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Sequencing of Short Peptides Using FAB Mass Spectrometry—Increased Information via Derivatization
Author(s) -
Renner Dieter,
Spiteller Gerhard
Publication year - 1985
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198504081
Subject(s) - derivatization , chemistry , mass spectrometry , fast atom bombardment , cleavage (geology) , peptide , chromatography , molecule , amino acid , peptide sequence , protein mass spectrometry , combinatorial chemistry , tandem mass spectrometry , organic chemistry , biochemistry , biology , fracture (geology) , gene , paleontology
Structure‐specific cleavage fragments, all of which contain the amino end of the molecule and which were formed by cleavage of the peptide bond, are obtained by fast atom bombardment (FAB) mass spectrometry of N ‐dansyl substituted small peptides. This derivatization thus allows an unequivocal determination of the amino acid sequence.