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Chemoenzymatic Synthesis of a Functional Gene for Human Gamma Interferon
Author(s) -
Engels Joachim,
Leineweber Michael,
Uhlmann Eugen
Publication year - 1984
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198409611
Subject(s) - gene , cloning (programming) , interferon , dna , biology , molecular cloning , interferon gamma , ligation , microbiology and biotechnology , chemistry , genetics , gene expression , cytokine , computer science , programming language
Chemoenzymatic gene synthesis in combination with cloning in E. coli enables the synthesis of larger proteins such as human gamma interferon. Chemical DNA synthesis followed by enzymatic coupling and cloning of the individual gene segments and ligation of the subfragments then yields the gene for the biologically active gamma interferon.