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Optimal Parameters in High Speed Liquid Chromatography (HPLC)
Author(s) -
Halász István,
Görlitz Gerhard
Publication year - 1982
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.198200501
Subject(s) - inlet , column chromatography , chromatography , bar (unit) , high performance liquid chromatography , two dimensional chromatography , misnomer , chromatography column , chemistry , supercritical fluid chromatography , column (typography) , separation (statistics) , phase (matter) , materials science , computer science , mathematics , geology , engineering , mechanical engineering , geometry , paleontology , oceanography , organic chemistry , machine learning , connection (principal bundle)
After a decade, the first storm of development in high performance liquid chromatography seems to be abating. Providing the stationary phase and eluent are optimized, most routine analytical and semi‐preparative separation problems require columns having between 1000 and 10000 theoretical plates. Commercial apparatus and columns completely satisfy these requirements. For many separation problems the efficiency of the columns on offer is greater than required. Since optimal (not maximal) separation is required with shorter analysis times, the column inlet pressure is increased in order to increase the eluent velocity, while at the same time reducing the column efficiency. The same result can be obtained by using shorter columns and lower pressures. Since inlet pressures of more than 100 bar are seldom required in routine analysis, the term “ high pressure liquid chromatography” is a misnomer.—It is probable that difficult separation problems, where several 100 000 theoretical plates are required, will be studied in the next phase of development. It can be shown using nomograms that such problems can be solved within acceptable time limits using inlet pressures of less than 500 bar. Further possible directions of development are indicated.